How much sample, in mg or ml of protein can I load onto my column? Affinity Chromatography The binding capacity values listed in the selection guide below, are typical for the given species. However, there might be considerable deviations in binding capacity for different immunoglobulins derivated from same species, even if they are of the same subclass. Protein binding capacity is protein-to-protein dependent. The given capacities are to be considered as starting values.
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HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G Superfine resin for fast and convenient desalting and buffer exchange. Convenient desalting and buffer exchange of sample volumes up to 1. Two to five columns can be connected in series for sample sizes from 3 mL to 7. Replaces dialysis for desalting protein, which saves time and reduces labor.
HiTrap column format is convenient for use with a syringe, a peristaltic pump, or a chromatography system. Column for desalting and buffer exchange of proteins HiTrap Desalting is a prepacked, ready-to-use column for group separation between high and low molecular weight substances. Applications include buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants, or removal of reagents to terminate a reaction. Read more The special design of the column, together with the well-known resin Sephadex G Superfine, provides fast and reproducible separations in a convenient format.
The exclusion limit is approximately Mr Successful use in published research HiTrap Desalting columns are used in a variety of research applications, including:.
HiTrap Desalting, 100 x 5 ml
HiTrap Desalting columns with Sephadex G-25 resin